rag1 (Cyagen Biosciences)

Cyagen Biosciences rag1

Fig. 1 GW4869 injected into asthmatic mice reduced the activation of ILC2s. a WT mice and <t>Rag1−/−mice</t> were sensitized via i.p. injection with 200 μg of papain on day 0 and 7, followed by i.n. administration of papain (100 μg) and GW4869 (1.5 mg/kg) on days 14–17. After the last treatment was completed, the mice were euthanized 24 h later, and samples were collected for analysis. b Flow cytometric analysis of lung ILC2s (CD45+Lin−CD90+ST2+); n = 4 mice. c The proliferation of ILC2s was indicated by Ki67 staining; n = 4 mice. d Flow cytometric analysis of IL-5 and IL-13 in ILC2s; n = 4 mice. e, f IL-5 (e) and IL-13 (f) concentrations (pg/ml) in BALF were determined by ELISA; n = 4 mice. g The frequencies of eosinophils (CD11c−Siglec F+) in BALF were analyzed by ﬂow cytometry; n = 4 mice. h, i Representative H&E staining (h) and PAS staining (i) of lung tissue sections from the indicated groups; n = 4 mice. The data are representative of two or three independent experiments and are presented as mean ± s.e.m. P values were calculated using two-sided Student’s t-tests or one-way ANOVA, followed by a multiple comparison test. ns not signiﬁcant, *P < 0.05, **P < 0.01 and ***P < 0.001.

Rag1, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rag1+ko/pm40451928-50-4-24?v=Cyagen+Biosciences
Average 94 stars, based on 1 article reviews

rag1 - by Bioz Stars, 2026-08

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mouse nod 129s7 b6 rag1 tm1mom j (Jackson Laboratory)

Jackson Laboratory mouse nod 129s7 b6 rag1 tm1mom j

IL-27 signaling in CD4 T cells impacts CD8 T cells (A) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. <t>Rag1</t> −/− recipients for analyzing Venus (IL-21)-expressing CD4 T cells. (B and C) IL-27 signaling in CD4 T cells promotes their IL-21 expression. Islet-infiltrating CD4 T cells were analyzed by flow cytometry for Venus (IL-21) expression at 8–9 weeks post-transfer. (B) Representative flow cytometry profiles of Venus (IL-21) and CXCR6 expression. (C) Summarized percentages of Venus (IL-21) + cells among CD4 T cells (upper) and CXCR6 + cells among Venus (IL-21) + CD4 T cells (lower). Combined results from two independent experiments are shown ( n = 5 per group). ∗∗ p < 0.01, unpaired t test. (D) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. Rag1 −/− recipients for analyzing the phenotype of CD8 T cells. (E) Representative histograms showing BATF expression in CD44 high CD8 T cells in the spleens (dotted lines) and pancreatic islets (shaded areas). gMFI, geometric mean fluorescent intensity. (F) Summarized BATF gMFI of spleen and islet CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 6–7 per group). ∗∗∗ p < 0.005, unpaired t test. (G) The BATF regulon activity in IGRP 206-241 -specific CD8 T cells isolated from pancreatic islets and spleens. The BATF regulon activity was computed by SCENIC using a previously published scRNA-seq dataset ( GSE200608 ). The p value is determined by Wilcoxon test. (H and I) IFNγ and TNFα expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. (H) Representative flow cytometry profiles of IFNγ and TNFα expression. (I) Summarized percentages of IFNγ + (left) and TNFα + (right) CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 5–6 per group). (J) Granzyme B expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. Representative histograms of granzyme B expression (left). Summarized relative granzyme B expression (right). Granzyme B gMFI of CD44 high CXCR6 + CD8 T cells co-transferred with Il27ra −/− CD4 T cells is normalized to those co-transferred with WT CD4 T cells in each experiment. Combined results from three independent transfer experiments are shown ( n = 10 per group). ∗ p < 0.05, unpaired t test.

Mouse Nod 129s7 B6 Rag1 Tm1mom J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rag1+ko/pmc12494592-64-0-10?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews

mouse nod 129s7 b6 rag1 tm1mom j - by Bioz Stars, 2026-08

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rag-1 crispr/cas9 ko plasmid (Santa Cruz Biotechnology)

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CRISPR/Cas9 KO Plasmids consists of RAG-1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)

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Fig. 1 GW4869 injected into asthmatic mice reduced the activation of ILC2s. a WT mice and Rag1−/−mice were sensitized via i.p. injection with 200 μg of papain on day 0 and 7, followed by i.n. administration of papain (100 μg) and GW4869 (1.5 mg/kg) on days 14–17. After the last treatment was completed, the mice were euthanized 24 h later, and samples were collected for analysis. b Flow cytometric analysis of lung ILC2s (CD45+Lin−CD90+ST2+); n = 4 mice. c The proliferation of ILC2s was indicated by Ki67 staining; n = 4 mice. d Flow cytometric analysis of IL-5 and IL-13 in ILC2s; n = 4 mice. e, f IL-5 (e) and IL-13 (f) concentrations (pg/ml) in BALF were determined by ELISA; n = 4 mice. g The frequencies of eosinophils (CD11c−Siglec F+) in BALF were analyzed by ﬂow cytometry; n = 4 mice. h, i Representative H&E staining (h) and PAS staining (i) of lung tissue sections from the indicated groups; n = 4 mice. The data are representative of two or three independent experiments and are presented as mean ± s.e.m. P values were calculated using two-sided Student’s t-tests or one-way ANOVA, followed by a multiple comparison test. ns not signiﬁcant, *P < 0.05, **P < 0.01 and ***P < 0.001.

Journal: Experimental & molecular medicine

Article Title: Extracellular vesicles derived from lung M2 macrophages enhance group 2 innate lymphoid cells function in allergic airway inflammation.

doi: 10.1038/s12276-025-01465-6

Figure Lengend Snippet: Fig. 1 GW4869 injected into asthmatic mice reduced the activation of ILC2s. a WT mice and Rag1−/−mice were sensitized via i.p. injection with 200 μg of papain on day 0 and 7, followed by i.n. administration of papain (100 μg) and GW4869 (1.5 mg/kg) on days 14–17. After the last treatment was completed, the mice were euthanized 24 h later, and samples were collected for analysis. b Flow cytometric analysis of lung ILC2s (CD45+Lin−CD90+ST2+); n = 4 mice. c The proliferation of ILC2s was indicated by Ki67 staining; n = 4 mice. d Flow cytometric analysis of IL-5 and IL-13 in ILC2s; n = 4 mice. e, f IL-5 (e) and IL-13 (f) concentrations (pg/ml) in BALF were determined by ELISA; n = 4 mice. g The frequencies of eosinophils (CD11c−Siglec F+) in BALF were analyzed by ﬂow cytometry; n = 4 mice. h, i Representative H&E staining (h) and PAS staining (i) of lung tissue sections from the indicated groups; n = 4 mice. The data are representative of two or three independent experiments and are presented as mean ± s.e.m. P values were calculated using two-sided Student’s t-tests or one-way ANOVA, followed by a multiple comparison test. ns not signiﬁcant, *P < 0.05, **P < 0.01 and ***P < 0.001.

Article Snippet: C57BL/6 female mice and Rag1−/− female mice, aged between 6 and 8 weeks and weighing 20–22 g, were sourced from GemPharmatech Co., Ltd. and Cyagen Biosciences Co., Ltd., respectively.

Techniques: Injection, Activation Assay, Staining, Enzyme-linked Immunosorbent Assay, Cytometry, Comparison

IL-27 signaling in CD4 T cells impacts CD8 T cells (A) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. Rag1 −/− recipients for analyzing Venus (IL-21)-expressing CD4 T cells. (B and C) IL-27 signaling in CD4 T cells promotes their IL-21 expression. Islet-infiltrating CD4 T cells were analyzed by flow cytometry for Venus (IL-21) expression at 8–9 weeks post-transfer. (B) Representative flow cytometry profiles of Venus (IL-21) and CXCR6 expression. (C) Summarized percentages of Venus (IL-21) + cells among CD4 T cells (upper) and CXCR6 + cells among Venus (IL-21) + CD4 T cells (lower). Combined results from two independent experiments are shown ( n = 5 per group). ∗∗ p < 0.01, unpaired t test. (D) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. Rag1 −/− recipients for analyzing the phenotype of CD8 T cells. (E) Representative histograms showing BATF expression in CD44 high CD8 T cells in the spleens (dotted lines) and pancreatic islets (shaded areas). gMFI, geometric mean fluorescent intensity. (F) Summarized BATF gMFI of spleen and islet CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 6–7 per group). ∗∗∗ p < 0.005, unpaired t test. (G) The BATF regulon activity in IGRP 206-241 -specific CD8 T cells isolated from pancreatic islets and spleens. The BATF regulon activity was computed by SCENIC using a previously published scRNA-seq dataset ( GSE200608 ). The p value is determined by Wilcoxon test. (H and I) IFNγ and TNFα expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. (H) Representative flow cytometry profiles of IFNγ and TNFα expression. (I) Summarized percentages of IFNγ + (left) and TNFα + (right) CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 5–6 per group). (J) Granzyme B expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. Representative histograms of granzyme B expression (left). Summarized relative granzyme B expression (right). Granzyme B gMFI of CD44 high CXCR6 + CD8 T cells co-transferred with Il27ra −/− CD4 T cells is normalized to those co-transferred with WT CD4 T cells in each experiment. Combined results from three independent transfer experiments are shown ( n = 10 per group). ∗ p < 0.05, unpaired t test.

Journal: iScience

Article Title: An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetes

doi: 10.1016/j.isci.2025.113537

Figure Lengend Snippet: IL-27 signaling in CD4 T cells impacts CD8 T cells (A) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. Rag1 −/− recipients for analyzing Venus (IL-21)-expressing CD4 T cells. (B and C) IL-27 signaling in CD4 T cells promotes their IL-21 expression. Islet-infiltrating CD4 T cells were analyzed by flow cytometry for Venus (IL-21) expression at 8–9 weeks post-transfer. (B) Representative flow cytometry profiles of Venus (IL-21) and CXCR6 expression. (C) Summarized percentages of Venus (IL-21) + cells among CD4 T cells (upper) and CXCR6 + cells among Venus (IL-21) + CD4 T cells (lower). Combined results from two independent experiments are shown ( n = 5 per group). ∗∗ p < 0.01, unpaired t test. (D) Schematic diagram of CD4 and CD8 T cell co-transfer into NOD. Rag1 −/− recipients for analyzing the phenotype of CD8 T cells. (E) Representative histograms showing BATF expression in CD44 high CD8 T cells in the spleens (dotted lines) and pancreatic islets (shaded areas). gMFI, geometric mean fluorescent intensity. (F) Summarized BATF gMFI of spleen and islet CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 6–7 per group). ∗∗∗ p < 0.005, unpaired t test. (G) The BATF regulon activity in IGRP 206-241 -specific CD8 T cells isolated from pancreatic islets and spleens. The BATF regulon activity was computed by SCENIC using a previously published scRNA-seq dataset ( GSE200608 ). The p value is determined by Wilcoxon test. (H and I) IFNγ and TNFα expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. (H) Representative flow cytometry profiles of IFNγ and TNFα expression. (I) Summarized percentages of IFNγ + (left) and TNFα + (right) CD44 high CD8 T cells. Combined results from two independent transfer experiments are shown (n = 5–6 per group). (J) Granzyme B expression in islet-infiltrating CD8 T cells co-transferred with WT or Il27ra −/− CD4 T cells. Representative histograms of granzyme B expression (left). Summarized relative granzyme B expression (right). Granzyme B gMFI of CD44 high CXCR6 + CD8 T cells co-transferred with Il27ra −/− CD4 T cells is normalized to those co-transferred with WT CD4 T cells in each experiment. Combined results from three independent transfer experiments are shown ( n = 10 per group). ∗ p < 0.05, unpaired t test.

Article Snippet: Mouse: NOD.129S7(B6)- Rag1 tm1Mom /J (NOD. Rag1 -/- ) , The Jackson Laboratory , RRID:IMSR_JAX:003729.

Techniques: Expressing, Flow Cytometry, Activity Assay, Isolation

IL-21 controls the effector functions of CD8 T cells (A) Schematic diagram of generating MBMCs. (B) Representative flow cytometry profiles showing the gating strategy for analyzing BATF expression in CD44 high CD8 T cells. Cells were stained with purified rabbit anti-mouse BATF primary antibody followed by fluorochrome-conjugated anti-rabbit IgG secondary antibody. The solid lines and shaded areas in the histograms, respectively, represent staining without and with the primary antibody. WT, wild type. gMFI: geometric mean fluorescent intensity. (C) Summarized BATF gMFI of spleen and islet CD44 high CD8 T cells. Combined results from two independent experiments are shown ( n = 6 per group). ∗ p < 0.05, paired t test. (D and E) The ability to produce IFNγ and TNFα is reduced in islet-infiltrating CD8 T cells in the absence of IL-21 signaling. (D) Representative flow cytometry profiles of IFNγ and TNFα expression. (E) Summarized percentages of IFNγ + (left) and TNFα + (right) CD44 high CD8 T cells. Combined results from three to four independent experiments are shown (n = 9–12 per group). ∗ p < 0.05, ∗∗ p < 0.01, paired t test. (F) Granzyme B expression in islet-infiltrating CD8 T cells is reduced in the absence of IL-21 signaling. Representative histogram of granzyme B expression (left). Summarized relative granzyme B expression (right). Granzyme B gMFI of Il21r −/− CD44 high CXCR6 + CD8 T cells is normalized to that of the WT counterpart in each MBMC experiment. Combined results from four independent experiments are shown ( n = 12 per group). ∗∗∗∗ p < 0.0001, paired t test. (G and H) Forced expression of BATF rescues the diabetogenic activity of CD8 T cells in the absence of CD4 T cell-derived IL-21. NY8.3 CD8 T cells were activated in vitro and transduced with either empty MIG or MIG-BATF retroviral vector (see ). A mixture of 5 × 10 6 transduced NY8.3 CD8 T cells along with 5 × 10 6 WT or Il21 −/− NOD CD4 T cells was transferred into NOD. Rag1 −/− female recipients. Mice were monitored for diabetes development. (G) The percentages of mice that developed diabetes over time are shown. Data are combined from two independent experiments with eight to nine total mice per group. ∗ p < 0.05, ∗∗∗ p < 0.001 by log rank test. (H) Representative islet images and the summary of insulitis in mice that remained nondiabetic at termination of the incidence study. Arrows indicate infiltrating immunocytes. Scale bars, 100 μm. ∗∗ p < 0.005, unpaired t test.

Journal: iScience

Article Title: An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetes

doi: 10.1016/j.isci.2025.113537

Figure Lengend Snippet: IL-21 controls the effector functions of CD8 T cells (A) Schematic diagram of generating MBMCs. (B) Representative flow cytometry profiles showing the gating strategy for analyzing BATF expression in CD44 high CD8 T cells. Cells were stained with purified rabbit anti-mouse BATF primary antibody followed by fluorochrome-conjugated anti-rabbit IgG secondary antibody. The solid lines and shaded areas in the histograms, respectively, represent staining without and with the primary antibody. WT, wild type. gMFI: geometric mean fluorescent intensity. (C) Summarized BATF gMFI of spleen and islet CD44 high CD8 T cells. Combined results from two independent experiments are shown ( n = 6 per group). ∗ p < 0.05, paired t test. (D and E) The ability to produce IFNγ and TNFα is reduced in islet-infiltrating CD8 T cells in the absence of IL-21 signaling. (D) Representative flow cytometry profiles of IFNγ and TNFα expression. (E) Summarized percentages of IFNγ + (left) and TNFα + (right) CD44 high CD8 T cells. Combined results from three to four independent experiments are shown (n = 9–12 per group). ∗ p < 0.05, ∗∗ p < 0.01, paired t test. (F) Granzyme B expression in islet-infiltrating CD8 T cells is reduced in the absence of IL-21 signaling. Representative histogram of granzyme B expression (left). Summarized relative granzyme B expression (right). Granzyme B gMFI of Il21r −/− CD44 high CXCR6 + CD8 T cells is normalized to that of the WT counterpart in each MBMC experiment. Combined results from four independent experiments are shown ( n = 12 per group). ∗∗∗∗ p < 0.0001, paired t test. (G and H) Forced expression of BATF rescues the diabetogenic activity of CD8 T cells in the absence of CD4 T cell-derived IL-21. NY8.3 CD8 T cells were activated in vitro and transduced with either empty MIG or MIG-BATF retroviral vector (see ). A mixture of 5 × 10 6 transduced NY8.3 CD8 T cells along with 5 × 10 6 WT or Il21 −/− NOD CD4 T cells was transferred into NOD. Rag1 −/− female recipients. Mice were monitored for diabetes development. (G) The percentages of mice that developed diabetes over time are shown. Data are combined from two independent experiments with eight to nine total mice per group. ∗ p < 0.05, ∗∗∗ p < 0.001 by log rank test. (H) Representative islet images and the summary of insulitis in mice that remained nondiabetic at termination of the incidence study. Arrows indicate infiltrating immunocytes. Scale bars, 100 μm. ∗∗ p < 0.005, unpaired t test.

Article Snippet: Mouse: NOD.129S7(B6)- Rag1 tm1Mom /J (NOD. Rag1 -/- ) , The Jackson Laboratory , RRID:IMSR_JAX:003729.

Techniques: Flow Cytometry, Expressing, Staining, Purification, Activity Assay, Derivative Assay, In Vitro, Transduction, Retroviral, Plasmid Preparation

Activated macrophages produce IL-27 in islets of NOD mice (A) Representative flow cytometry profiles showing the gating strategy for identifying GFP (IL-27p28)-expressing cells in the islets of NOD. Il27p28-eGFP mice. Gating of GFP (IL-27p28) + cells was based on an Il27p28-eGFP -negative mouse. (B) The frequencies of GFP (IL-27p28) + cells summarized from two independent experiments using 9- to 12-week-old females are shown ( n = 8). (C) Correlation between the frequencies of CD3 + T cells and GFP (IL-27p28) + macrophages in 5- to 12-week-old NOD. Il27p28-eGFP females. Each symbol represents one mouse. (D and E) Percentages of GFP (IL-27p28) + cells in (D) islet and (E) splenic CD11c + F4/80 + and CD11c low F4/80 + cells of 11-week-old wild-type (WT) and 17-week-old Rag1 −/− NOD. Il27p28-eGFP female mice ( n = 4 per group). ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, unpaired t test. ns, not significant. (F) Percentages of total Il27 + (left), total Ebi3 + (middle), and Il27 + Ebi3 + (right) cells among islet macrophages. (G) Proportions of different islet macrophage subpopulations among total Il27 + (left), total Ebi3 + (middle), and Il27 + Ebi3 + (right) cells. Results from (F and G) were generated using a previously published scRNA-seq dataset ( GSE141786 ). (H) Representative flow cytometry profiles showing the expression of GFP (IL-27p28) in activated (CD40 high ) F4/80 + islet macrophages of WT and Rag1 −/− NOD. Il27p28-eGFP mice.

Journal: iScience

Article Title: An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetes

doi: 10.1016/j.isci.2025.113537

Figure Lengend Snippet: Activated macrophages produce IL-27 in islets of NOD mice (A) Representative flow cytometry profiles showing the gating strategy for identifying GFP (IL-27p28)-expressing cells in the islets of NOD. Il27p28-eGFP mice. Gating of GFP (IL-27p28) + cells was based on an Il27p28-eGFP -negative mouse. (B) The frequencies of GFP (IL-27p28) + cells summarized from two independent experiments using 9- to 12-week-old females are shown ( n = 8). (C) Correlation between the frequencies of CD3 + T cells and GFP (IL-27p28) + macrophages in 5- to 12-week-old NOD. Il27p28-eGFP females. Each symbol represents one mouse. (D and E) Percentages of GFP (IL-27p28) + cells in (D) islet and (E) splenic CD11c + F4/80 + and CD11c low F4/80 + cells of 11-week-old wild-type (WT) and 17-week-old Rag1 −/− NOD. Il27p28-eGFP female mice ( n = 4 per group). ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, unpaired t test. ns, not significant. (F) Percentages of total Il27 + (left), total Ebi3 + (middle), and Il27 + Ebi3 + (right) cells among islet macrophages. (G) Proportions of different islet macrophage subpopulations among total Il27 + (left), total Ebi3 + (middle), and Il27 + Ebi3 + (right) cells. Results from (F and G) were generated using a previously published scRNA-seq dataset ( GSE141786 ). (H) Representative flow cytometry profiles showing the expression of GFP (IL-27p28) in activated (CD40 high ) F4/80 + islet macrophages of WT and Rag1 −/− NOD. Il27p28-eGFP mice.

Article Snippet: Mouse: NOD.129S7(B6)- Rag1 tm1Mom /J (NOD. Rag1 -/- ) , The Jackson Laboratory , RRID:IMSR_JAX:003729.

Techniques: Flow Cytometry, Expressing, Generated

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